Categories

Serum Bilirubin:

1-Total Bilirubin:

Procedure:
sample serum or plasma

DON'T HAEMOLYSIS

1. Prepare two test tubes mark one as Blank tube and the other as Test tube .
2. put in the blank tube : 100 µ reagent 1 + 500 µ reagent 3 + 100 µ specimen mix and leave for 10 min. at room temp.
3. at same time put in test tube : 100 µ reagent 1 + 25 µ reagent 2 + 500 µ reagent 3 + 100 µ specimen mix and leave for 10 min. in room temp.
4. then put 500 µ reagent 4 in each tube mix and leave for 5 min. in room temp.
5. MEASURING AGAINST BLANK at wave length 580 nm.

Calculation:
reading of test x 10.8 = ......mg / dl .

Normal values up to 1.0 mg / dl .

2-Direct Bilirubin:

1. Prepare two test tubes mark one as Blank tube and the other as Test tube .
2. put in the blank tube : 100 µ reagent 1 + 1 ml saline + 100 µ specimen mix and leave for 5 min.
3. put in test tube at same time : 100 µ reagent 1 + 25 µ reagent 2 + 1 ml saline + 100 µ specimen mix and leave for 5 min.
4. MEASURE AGAINST BLANK at wave length 580 nm .

Calculation:
Reading of test x 14.4 = ....... mg / dl .

Normal values up to 0.25 mg / dl .

تحليل الصفراء فى الدم

Total Bilirubin
تطلب فى حالات الاطفال حديثى الولادة حيث يغلب حدوث الصفراء الفسيولوجية

ملاحظات علاجية : ينصح بتعرض الاطفال فى هذه الحالة الى الضوء وايضا ينصح بتناول الام كمية كبيرة من السكريات

اعراض الصفراء : ارتفاع فى درجات الحرارة تغيير لون العين الى المصفر الم بالبطن وايضا يلاحظ تغير بلون البول

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Comments

	Amin Gamal Amin
Thank u

	dr_ch\ alihamdey
thank uuuuuuuuu . http://www.khayma.com/keemia-web/photo1/belflgoL.gif

	alihamdey
Thank u

	zahrat elmadaen
thank you very much

	zahrat elmadaen
THANK YOU ALOT ABOUT THIS INFORMATION

	shaza
thank you

	سحرعبده
ماشاء الله معلومات مبسطة جزاكم الله خيرا

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