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Serum Bilirubin:
1-Total Bilirubin:
Procedure:
sample serum or plasma
DON'T HAEMOLYSIS
1. Prepare two test tubes mark one as Blank tube and the other as Test tube .
2. put in the blank tube : 100 µ reagent 1 + 500 µ reagent 3 + 100 µ specimen mix and leave for 10 min. at room temp.
3. at same time put in test tube : 100 µ reagent 1 + 25 µ reagent 2 + 500 µ reagent 3 + 100 µ specimen mix and leave for 10 min. in room temp.
4. then put 500 µ reagent 4 in each tube mix and leave for 5 min. in room temp.
5. MEASURING AGAINST BLANK at wave length 580 nm.
Calculation:
reading of test x 10.8 = ......mg / dl .
Normal values up to 1.0 mg / dl .
2-Direct Bilirubin:
1. Prepare two test tubes mark one as Blank tube and the other as Test tube .
2. put in the blank tube : 100 µ reagent 1 + 1 ml saline + 100 µ specimen mix and leave for 5 min.
3. put in test tube at same time : 100 µ reagent 1 + 25 µ reagent 2 + 1 ml saline + 100 µ specimen mix and leave for 5 min.
4. MEASURE AGAINST BLANK at wave length 580 nm .
Calculation:
Reading of test x 14.4 = ....... mg / dl .
Normal values up to 0.25 mg / dl .
تحليل الصفراء فى الدم
Total Bilirubin
تطلب فى حالات الاطفال حديثى الولادة حيث يغلب حدوث الصفراء الفسيولوجية
ملاحظات علاجية : ينصح بتعرض الاطفال فى هذه الحالة الى الضوء وايضا ينصح بتناول الام كمية كبيرة من السكريات
اعراض الصفراء : ارتفاع فى درجات الحرارة تغيير لون العين الى المصفر الم بالبطن وايضا يلاحظ تغير بلون البول
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 | Amin Gamal Amin |
Thank u | |
 | dr_ch\ alihamdey |
thank uuuuuuuuu . | |
| alihamdey |
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 | zahrat elmadaen |
thank you very much | |
| zahrat elmadaen |
THANK YOU ALOT ABOUT THIS INFORMATION | |
| shaza |
thank you | |
| سحرعبده |
ماشاء الله معلومات مبسطة | |
| korshy |
جزاكم الله كل خير mkorshy@yahoo.com | |
| nelrayes |
بسم الله الرحمن الرحيم | |
 | amon |
ازا بتقدرو تحطو شو مكونات كل reagent | |
