Indirect Antiglobulin Test (Indirect Coomb's test)
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The sweat test measures the amount of sodium and chloride in sweat. Sodium and chloride are part of your body’s electrolyte balance and combine to form the salt found in sweat.
They help regulate the fluid balance in tissues. Normally, chloride travels in and out of the body’s cells, helping to maintain electrical neutrality and water balance.
This movement occurs through a protien, the cystic fibrosis transmembrane conductance regulator (CFTR), that serves as a channel, letting chloride out of cells and into the surrounding fluid and also reducing sodium absorption. Sodium levels thus usually mirror those of chloride.
Methods of sweat analysis
Two methods of sweat analysis are most frequently used: chloride concentration and conductivity measurement. The sweat chloride analysis is recommended as the diagnostic test for CF. Sweat conductivity may be used to screen for CF.
اختبار العرق يقيس كمية الكلوريد والصوديوم الموجودة بالعرق وهما المكونان الرئيسيين للملح الموجود بالعرق وهذه الاملاح تعمل على حفظ سوائل الجسم عن طريق تفككها وخروج الكلوريد من الانسجة فيحدث التوازن