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Random articles

Gram stain procedure

ØÑíÞÉ ÕÈÛÉ ÌÑÇã (ÕÈÛÉ ÛÑÇã)

This stain for differentiate between G +ve bacteria (which accept staining) and G -ve bacteria (which not accept staining).

Steps:
-Prepare your clear slide and put one drop of normal saline.
-Take specimen from the colony and mix it well with normal saline.
-Leave for dryness on air then fix it on burner.
-Then pour Crystal Violet stain on the slide and leave it for 1 minute.
-Wash gently with water.
-Then pour Iodine stain on the slide and leave it for 1 minute.
-Wash gently w...Read more

Antibiotic Susceptibility Test

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This test for testing number of antibiotics against certain organism to find the best one and the most effective on this organism.

Steps:
-Take a swap from the colony by a sterile wire loop and culture it on Muller-hinton agar.
-Leave it incubated for 24 hours at 37ºC.

Result:
You notice now the larger clear zone around an antibiotic means that this antibiotic is the best.
See Samples below......Read more

Polymerase Chain Reaction (PCR)

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ÊÝÇÚá ÇáÈæáíãÑíÒ ÇáãÊÓáÓá

ÊÞæã ÝßÑÉ ÊÞäíÉ Çá PCR Åáì ÇáÞíÇã ÈÊÖÎíã ÌÒíÆÇÊ ÞáíáÉ ãä ÇáÍãÖ Çáäææí DNA¡ ÈÚÏ ÇÓÊÎáÇÕå ãä ÎáÇíÇ Ãæ ÓæÇÆá ÇáÌÓã æÈÇáÊÇáí ÇáÍÕæá Úáì ßãíÇÊ ßÈíÑÉ ãäå æÇáÊì ÊãßääÇ ãä ÅÌÑÇÁ ÇáÊÍáíá Úáíå. íãßä ÇÚÊÈÇÑ ÊÞäíÉ PCR ÊÑÌãÉ ãÈÓØÉ áÚãáíÉ ÇäÊÓÇÎ ÇáÍãÖ Çáäææí DNA ÃËäÇÁ ÇáÇäÞÓÇã ÇáÎáæí.æáßí íÊã åÐÇ ÇáÇäÊÓÇΡ áÇ ÈÏ ãä ÊæÝÑ ãæÇÏ ãÚíäÉ ÊÓÇÚÏ Úáì Ðáß:

1. ÇáÈÇÏÆÇÊ Primers æåí ÚÈÇÑÉ Úä äíæßáæÊíÏÇÊ ÞáíáÉ Oligonucleotides18 – 20 ÃÓÇÓ ÂÒæÊí ÞÇÏÑÉ Úáì Ç...Read more