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ÊÞæã ÝßÑÉ ÊÞäíÉ Çá PCR Åáì ÇáÞíÇã ÈÊÖÎíã ÌÒíÆÇÊ ÞáíáÉ ãä ÇáÍãÖ Çáäææí DNA¡ ÈÚÏ ÇÓÊÎáÇÕå ãä ÎáÇíÇ Ãæ ÓæÇÆá ÇáÌÓã æÈÇáÊÇáí ÇáÍÕæá Úáì ßãíÇÊ ßÈíÑÉ ãäå æÇáÊì ÊãßääÇ ãä ÅÌÑÇÁ ÇáÊÍáíá Úáíå. íãßä ÇÚÊÈÇÑ ÊÞäíÉ PCR ÊÑÌãÉ ãÈÓØÉ áÚãáíÉ ÇäÊÓÇÎ ÇáÍãÖ Çáäææí DNA ÃËäÇÁ ÇáÇäÞÓÇã ÇáÎáæí.æáßí íÊã åÐÇ ÇáÇäÊÓÇΡ áÇ ÈÏ ãä ÊæÝÑ ãæÇÏ ãÚíäÉ ÊÓÇÚÏ Úáì Ðáß:
1. ÇáÈÇÏÆÇÊ Primers æåí ÚÈÇÑÉ Úä äíæßáæÊíÏÇÊ ÞáíáÉ Oligonucleotides18 – 20 ÃÓÇÓ ÂÒæÊí ÞÇÏÑÉ Úáì Ç...Read more
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ÒÑÇÚÉ ÇÎÊÈÇÑ ÞæÉ ÇáãÖÇÏÇÊ ÇáÍíæÉ
This test for testing number of antibiotics against certain organism to find the best one and the most effective on this organism.
Steps:
-Take a swap from the colony by a sterile wire loop and culture it on Muller-hinton agar.
-Leave it incubated for 24 hours at 37ºC.
Result:
You notice now the larger clear zone around an antibiotic means that this antibiotic is the best.
See Samples below......Read more
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ÇáßÔÝ Úä ÝÕíáÉ ÇáÏã
Sample is fresh blood or EDTA blood(anticoagulant)
Put 10 µ of anti A on one side of a slide and put 10 µ of anti B on the other side
Put 10 µ of blood tested in each side and mix the blood with the reagent added.
results:
+ A & + B = AB
+ A & - B = A
- A & + B = B
-A & - B = O ...Read more
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