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Random articles

Reticulocytes Test

ÇÎÊÈÇÑ ÇáÎáÇíÇ ÇáÔÈßíÉ


ÇÎÊÈÇÑ ÇáÎáÇíÇ ÇáÔÈßíÉ

ÊÍÖíÑ ÇáÕÈÛÉ:

-ÊÐÇÈ ÈæÏÑÉ ÇÒÑÞ ÇááãÇÚ ÈãÞÏÑÇ 1 Ìã Ýì 100 ãáì ãÍáæá ãßæä ãä 80 ãáì ãÍáæá ãáÍì(9 Ìã ãáÍ Ýì ÇááÊÑ) æ 20 ãáì ãÍáæá ÓÊÑÇÊ ÕæÏíæã 3.1 %.Ëã íÑÔÍ ÇáãÍáæá ÈÚÏ ÊÍÖíÑå.
-ÊÕÈÛ ÇáÎáíÉ ÇáÔÈßíÉ ÈÇáÇÒÑÞ ÇááãÇÚ ÍÊì íãßä ÊãííÒåÇ Úä ÈÇÞì ßÑÇÊ ÇáÏã ÇáÍãÑÇÁ.


ÇáØÑíÞÉ:

-äÖÚ Ýì ÇäÈæÈÉ æÇÒÑãÇä 3 äÞØ ãä ÕÈÛÉ ÇáÇÒÑÞ ÇááãÇÚ.
-íÖÇÝ ÖÚÝåÇ Ïã Çì 6 äÞØ Ïã ãÃÎæÐÉ Úáì ãÇäÚ ÊÌáØ Ýì ÇáÍÇáÇÊ ÇáÚÇÏíÉ ÃãÇ Ýì ÍÇáÇÊ ÇáÇäíãíÇÁ äÖíÝ 1...Read more

Gram stain procedure

ØÑíÞÉ ÕÈÛÉ ÌÑÇã (ÕÈÛÉ ÛÑÇã)

This stain for differentiate between G +ve bacteria (which accept staining) and G -ve bacteria (which not accept staining).

Steps:
-Prepare your clear slide and put one drop of normal saline.
-Take specimen from the colony and mix it well with normal saline.
-Leave for dryness on air then fix it on burner.
-Then pour Crystal Violet stain on the slide and leave it for 1 minute.
-Wash gently with water.
-Then pour Iodine stain on the slide and leave it for 1 minute.
-Wash gently w...Read more

Antibiotic Susceptibility Test

ÒÑÇÚÉ ÇÎÊÈÇÑ ÞæÉ ÇáãÖÇÏÇÊ ÇáÍíæÉ

This test for testing number of antibiotics against certain organism to find the best one and the most effective on this organism.

Steps:
-Take a swap from the colony by a sterile wire loop and culture it on Muller-hinton agar.
-Leave it incubated for 24 hours at 37ºC.

Result:
You notice now the larger clear zone around an antibiotic means that this antibiotic is the best.
See Samples below......Read more